Department of Medicine

Department of Medicine

 Division of Renal-Electrolyte

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photo Shujie Shi, PhD

Research Instructor


Phone: 412-383-9569

Office: 3550 Terrace Street
929 Scaife Hall
Pittsburgh, PA 15261
Phone: 412-383-9569
Fax: 412-383-8956
Education and Training
PhD, University of Rochester, 2010
M.S., Nankai University, China, 2002
B.S., Nankai University, China, 1999
Postdoctoral Fellow, University of Pittsburgh, 2013
Research Interest
Mechanically gated ion channels play essential roles in transforming mechanical forces into cellular signals, a biological process referred to as mechanosensation. The focus of Dr. Shi’s research is to explore mechanisms by which ion channels of the epithelial sodium channel (ENaC)/degenerin family are regulated by mechanical forces. She uses the two expression systems, Xenopus oocytes and C. elegans worms, to perform systematic structure-function studies, and then translates her findings into a whole animal setting. She discovered that the C. elegans degenerin channel was activated by shear stress and the two pore-forming subunits, MEC-4 and MEC-10 had distinct roles in this response. She is currently working on identifying key domain or sites within the degenerin channel required for the channel’s activation by shear stress. She is also studying how accessory proteins, such as MEC-6 and its mammalian homology PON-2 regulate the channel activity and gating.
For my complete bibliography, Click Here.
Selected Publications:
Shujie Shi, Thomas R. Kleyman. Gamma subunit second transmembrane domain contributes to epithelial sodium channel gating and amiloride block. American Journal of Physiology - Renal Physiology. 2013; 305(11): 1585-1592.
Shujie Shi, Marcelo D. Carattino, Rebecca P. Hughey, Thomas R. Kleyman. ENaC regulation by proteases and shear stress. Current Molecular Pharmacology. 2013; 6 (1): 28-34.
Shujie Shi, Marcelo D. Carattino, Thomas R. Kleyman. Role of the Wrist Domain in the Response of the Epithelial Sodium Channel to External Stimuli. Journal of Biological Chemistry. 2012; 287(53): 44027-44035.
Shujie Shi, Brandon M. Blobner, Ossama B. Kashlan, Thomas R. Kleyman. Extracellular finger domain modulates the response of the epithelial sodium channel to shear stress. Journal of Biological Chemistry. 2012; 287(19): 15439-15444.
Shujie Shi, Dipon D. Ghosh, Sora Okumura, Marcelo D. Carattino, Ossama B. Kashlan, Shaohu Sheng, Thomas R. Kleyman. Base of the thumb domain modulates epithelial sodium channel gating. Journal of Biological Chemistry. 2011; 286(17): 14753-14761.
Tania Abi-Antoun, Shujie Shi, Lindsey A. Tolino, Thomas R. Kleyman, Marcelo D. Carattino. Second transmembrane domain modulates epithelial sodium channel gating in response to shear stress. American Journal of Physiology - Renal Physiology. 2011; 300(5): 1089-1095.
Shujie Shi, Cliff J. Luke, Mark T. Miedel, Gary A. Silverman, Thomas R. Kleyman. Activation of the Caenorhabditis elegans degenerin channel by shear stress requires the MEC-10 subunit. The Journal of Biological Chemistry. 2016.
Sponsored Research/Activities
Title: Regulation of ENaC/Degenerin Channels by Mechanical Forces
Role: Principal Investigator
Funding Agency: National Institute of Diabetes, Digestive, & Kidney Disease
Grant Number: K01 DK103834
Start Year: 2014
End Year: 2019