Department of Medicine

Department of Medicine

 Division of Infectious Diseases

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photo Zandrea Ambrose, PhD


Phone: 412-624-0512

Office: S830 Scaife Hall
3550 Terrace Street
Pittsburgh, PA 15261
Phone: 412-624-0512
Fax: 412-648-8521
Education and Training
BA, Ohio Wesleyan University, 1994
PhD, University of Washington, 2001
Research fellow, HIV Drug Resistance Program, National Cancer Institute, 2007
Research Interest
The Ambrose laboratory takes three approaches to studying HIV infection and therapeutics. Half of the laboratory focuses on basic molecular and cellular retrovirology, while the other half uses animal models to address important questions about HIV infection, pathogenesis, prevention, and treatment.
Educational Interest
Dr. Ambrose has mentored undergraduate, graduate, and post-graduate students in her laboratory. She is a member of the graduate faculty in the School of Medicine Interdisciplinary Biomedical Graduate Program and in the Graduate School of Public Health, Department of Infectious Diseases and Microbiology. She teaches in several graduate virology courses and in the Medical Microbiology course for first year medical students. Dr. Ambrose also participates in several student journal clubs and judges regional and international student science and engineering fairs.
For my complete bibliography, Click Here.
Selected Publications:
Kearney MF, Anderson EM, Coomer C, Smith L, Shao W, Johnson N, Kline C, Spindler J, Mellors JW, Coffin JM, Ambrose Z. Well-mixed plasma and tissue viral populations in RT-SHIV-infected macaques implies a lack of viral replication in the tissues during antiretroviral therapy. Retrovirology. 2015; 12: 93.
Melody K, McBeth S, Kline C, Kashuba ADM, Mellors JW, Ambrose Z. Low frequency of drug-resistant variants selected by long-acting rilpivirine (RPV LA) in macaques infected with RT-SHIV. Antimicrobial Agents Chemotherapy. 2015; 59: 7762-70.
Kline C, Ndjomou J, Franks T, Kiser R, Coalter V, Smedley J, Piatek M, Mellors JW, Lifson JD, Ambrose Z. Persistence of viral reservoirs in multiple tissues after ART suppression in a macaque RT-SHIV model. PLoS One. 2013; 8: e84275.
Xu H, Franks T, Gibson G, Huber K, Rahm N, Strambio de Castillia C, Luban J, Aiken C, Watkins S, Sluis-Cremer N, Ambrose Z. Evidence for bi-phasic uncoating during HIV-1 infection from a novel imaging assay. Retrovirology. 2013; 10: 70.
Ambrose Z., Lee K, Ndjomou J, Xu H, Oztop I, Matous J, Takemura T, Unutmaz D, Engelman A, Hughes SH, KewalRamani VN. Human immunodeficiency virus type 1 capsid mutation N74D alters cyclophilin A dependence and impairs microphage infection. Journal of Virology. 2012; 86: 4708-14.
Kearney M, Spindler J, Shao W, Maldarelli F, Palmer S, Hu SL, Lifson JD, KewalRamani VN, Mellors JW, Coffin JM, Ambrose Z. Genetic diversity of simian immunodeficiency virus encoding HIV-1 reverse transcriptase (RT-SHIVmne) persists in macaques despite antiretroviral therapy. Journal of Virology. 2012; 85: 1067-76.
Sponsored Research/Activities
Title: Visualization of In Vivo HIV-1 Vaginal Transmission in the Presence and Absence of PrEP
Role: Principal Investigator
Funding Agency: National Institute of Allergy & Infectious Diseases
Grant Number: R01 AI116276
Start Year: 2014
End Year: 2019
Title: Pittsburgh Center for HIV Protein Interactions (PCHPI)
Role: Principal Investigator
Funding Agency: National Institute of General Medical Science
Grant Number: P50 GM082251
Start Year: 2012
End Year: 2017
Title: Origin and Evolution of HIV-1 Drug Resistance in the RT-SHIV Macaque Model
Role: Principal Investigator
Funding Agency: National Institute of Allergy & Infectious Diseases
Grant Number: R01 AI080290
Start Year: 2010
End Year: 2014
Title: Host and Pathogen Diversity in Mtb and SIV Infection
Role: Co-Investigator
Funding Agency: National Institute of Allergy & Infectious Diseases
Grant Number: R21 AI127066
Start Year: 2016
End Year: 2018
Title: Visualizing the Dynamics of HIV Reseravoirs in Response to Antiretroviral Therapy
Role: Co-Investigator
Funding Agency: National Institute of Mental Health
Grant Number: R21 MH100949
Start Year: 2013
End Year: 2015
Title: Mechanisms of HIV Drug Resistance
Role: Co-Investigator
Funding Agency: Leidos Biomedical Research, Inc./National Cancer Institute
Start Year: 2012
End Year: 2016