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Department of Medicine

Department of Medicine

 Division of Renal-Electrolyte

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photo Ossama B. Kashlan, PhD

Assistant Professor of Medicine

Email: obk2@pitt.edu

Phone: 412-648-9277

Contact
Office: S828B Scaife Hall
3550 Terrace St.
Pittsburgh, PA 15261
 
Phone: 412-648-9277
Fax: 412-383-8956
E-mail: obk2@pitt.edu
Education and Training
Education
B.A., University of Pennsylvania, 1997
Ph.D., University of Pennsylvania, 2002
Training
Postdoctoral Fellow, University of Pittsburgh, 2006
Research Interest
Dr. Kashlan's research efforts are focused on the study of epithelial ion channels. His research team seeks a structurally based understanding of the mechanisms of regulation of this channel, e.g. activation by proteases or inhibition by sodium. His lab combines functional characterization by electrophysiology with x-ray crystallography and molecular modeling to study structure. Complementary to these approaches, his lab also employs molecular biology and molecular evolution to gather further insight into the molecular mechanisms of channel regulation.
Publications
For my complete bibliography, Click Here.
Selected Publications:
Kashlan OB, Adelman JL, Okumura S, Blobner BM, Zuzek Z, Hughey RP, Kleyman TR, Grabe M. Constraint-based, homology model of the extracellular domain of the epithelial Na+ channel a subunit reveals a mechanism of channel activation by proteases. J Biol Chem. 2011; 286: 649-60.
Shi S, Ghosh DD, Okumura S, Carattino MD, Kashlan OB, Sheng S, Kleyman TR. Base of the thumb domain modulates epithelial sodium channel gating. J Biol Chem. 2011; 286: 14753-61.
Tolino LA, Okumura S, Kashlan OB, Carattino MD. Insights into the Mechanism of Pore Opening of Acid-sensing Ion Channel 1A. J Biol Chem. 2011; 286: 16297-307.
Kashlan OB, Boyd CR, Argyropoulos C, Okumora S, Hughey RP, Grabe M, Kleyman TR. Allosteric inhibition of the epithelial Na+ channel through peptide binding at peripheral finger and thumb domains. J Biol Chem. 2010; In Press.
Mueller GM, Maarouf AB, Kinlough CL, Sheng N, Kashlan OB, Okumura S, Luthy S, Kleyman TR, Hughey RP. Cys-palmitoylation of the beta subunit modulates gating of the epithelial sodium channel. J Biol Chem. 2010; 285: 30453-62.
Passero CJ, Carattino MD, Kashlan OB, Myerburg MM, Hughey RP, Kleyman TR. Defining an inhibitory domain in the gamma subunit of the epithelial sodium channel. Am J Physiol Renal Physiol. 2010; 299: 854-61.
Mueller GM, Kashlan OB, Bruns JB, Maarouf AB, Aridor M, Kleyman TR, Hughey RP. Epithelial sodium channel exit from the endoplasmic reticulum is regulated by a signal within the carboxyl cytoplasmic domain of the alpha subunit. J Biol Chem. 2007; 282: 33475-83.
Kashlan OB, Mueller GM, Qamar MZ, Poland PA, Ahner A, Rubenstein RC, Hughey RP, Brodsky JL, Kleyman. Small heat shock protein aA-crystallin regulates epithelial sodium channel expression. J Biol Chem. 2007; 282: 28149-56.
Kashlan OB, Maarouf AB, Kussius C, Denshaw RM, Blumenthal KM, Kleyman TR. Distinct structural elements in the first membrane-spanning segment of the epithelial sodium channel. J Biol Chem. 2006; 281: 30455-62.
Goldfarb S, Kashlan OB, Watkins J, Suaud L, Kleyman TR, Rubenstein RC. Differential Effects of Hsc70 and Hsp70 on the Intracellular Trafficking and Functional Expression of Epithelial Sodium Channels. Proc Natl Acad Sci USA. 2006; 103: 5817-22.
Sponsored Research/Activities
Title: Allosteric ENaC Regulation
Role: Principal Investigator
Funding Agency: National Institute of Diabetes, Digestive, & Kidney Disease
Grant Number: R01 DK098204
Start Year: 2014
End Year: 2019
Title: Refining a Structural Model of ENaC
Role: Principal Investigator
Funding Agency: National Institute of Diabetes, Digestive, & Kidney Disease
Grant Number: R03 DK 099247
Start Year: 2013
End Year: 2014
Title: Organization and Structure of ENaC Transmembrane Segments
Role: Principal Investigator
Funding Agency: National Institute of Diabetes, Digestive, & Kidney Disease
Grant Number: K01 DK078734
Start Year: 2008
End Year: 2014