Dr. DeLany’s research interests include: (1) the role of energy expenditure, physical activity and energy intake in the development of obesity and in effective weight loss, and changes during and after weight loss through bariatric surgery and behavioral intervention; 2) understanding the relation between skeletal muscle characteristics, mitochondrial function, substrate utilization, body composition and insulin sensitivity; and (3) examining metabolic factors related to the increased risk of development of obesity and diabetes in African-American women. In studies of energy metabolism and physical activity, state of the art methodology is applied, including longitudinal measures of body composition by dual energy x-ray absorptiometry, and total energy expenditure by doubly labeled water, assessment of resting metabolic rate and substrate utilization by indirect calorimetry, and the use of multisensor physical activity monitors. For examination of insulin sensitivity, state of the art methodology, including hyperinsulinemic, euglycemic clamps with stable isotope glucose tracer to differentiate between liver and peripheral insulin sensitivity, skeletal muscle biopsies, high resolution respirometry, histology and electron microscopy are utilized. Ongoing and planned studies will include examination of the role mitochondrial genetics in insulin sensitivity and positron emission tomography studies of skeletal muscle and adipose tissue glucose metabolism. In addition, Dr. DeLany provides scientific leadership and technical expertise for the Mass Spectrometry Lab to be able to function as a “core laboratory” for stable isotope methodologies and quantification of blood and tissue compounds for investigators within the University of Pittsburgh community as well as outside investigators who need these services.